Biosynthesis and Characterization of Silver Nanoparticles Mediated by Extracellular Fungal Filtrate

By : Aadity Saitawadekar , Umesh B. Kakde

Page No: 403-409

Abstract
The two basic approaches to manufacture NPs are the top-down and bottom-up techniques. Bottom-up approach is the alternative approach to the physical method of synthesis of NPs, which has the potential of creating less waste and hence being more economical. Nanoparticles from green synthesis have been getting an increasing advantage due to it being a cost effective and eco-friendly method for nanomaterial synthesis. The present investigation focuses on the biological synthesis, characterization of biogenic nanoparticles using extracellular filtrate of Aspergillus flavus. The characterization of synthesised silver nanoparticles has been done using techniques such as UV-Vis Spectroscopy, Fourier transform infrared (FTIR), Transmission electron microscopy (TEM) and Nanoparticle tracking analysis (NTA). The maximum peak was observed at 426 nm in UV-vis spectroscopy after every 5 minutes. In order to identify the presence of functional groups in extracellular fungal filtrate which was used for bioreduction of silver and capping of silver nanoparticles, FTIR analysis has been carried out. The spherical aggregate shaped silver nanoparticles synthesized by A. flavus ranged from 7-50 nm confirmed in TEM as well as in NTA.

Authors :
Aadity Saitawadekar and Umesh B. Kakde
Department of Botany, The Institute of Science, Mumbai, India.
 

DOI: https://doi.org/10.32381/JPSR.2022.38.01.43