Phytochemical Analysis, Antioxidant and Antimicrobial Potential of n-butanol Fraction of Ethanolic Extract of A. muricata L. Leaves

By : Haripriya. R, P. Thirumalai Vasan

Page No: 365-375

Abstract 
The tropical plant, Annona muricata has diverse health benefits and exhibits pharmacological activities such as antimicrobial, antioxidant and anticancer properties. The n-butanol fraction of the ethanolic leaf extract was derived from the plant using Soxhlet-extraction which is followed by qualitative and quantitative determination of phytoconstituents present in the leaf extract. The qualitative determination reveals the primary and secondary metabolites where anthraquinone was reported to be absent. The leaf extract was subjected to antimicrobial activity using Agar well diffusion assay which confirmed the antibacterial and antifungal property against Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus niger, Candida albicans respectively. The free radical scavenging property (antioxidant activity) of the phytochemicals present in the leaf extract was confirmed by DPPH assay and maximum inhibition of 87% of free radicals were observed at lowest concentration (100 µg/mL) and the IC50 value was found to be 59.72 µg/mL showing 50% of inhibition. The HPLC and GCMS analysis of the n-butanol fraction of ethanol extract of leaf confirmed the high abundance of phytoconstituents required for antioxidant and antimicrobial activity.

Authors :
Haripriya. R : Research scholar, PG and Research Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, T.V.Kovil, Trichy.
P. Thirumalai Vasan : Head, PG and Research Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Affiliated to Bharathidasan University, T.V. Kovil, Trichy.
 

DOI: https://doi.org/10.32381/JPSR.2022.38.01.39