Peptone Induced Pigment Production of Ganoderma lucidum

By : Mona Kejariwal, Sakshi Soni, Saloni Koli, Eeshrita Jog, Sayok Ghosh, Deepak Singh, Surabhi Parker, Tanvi Patil, Khushboo Chouhan, Akash Kushwaha, Kanika Shetty

Page No: 165-172

Abstract
Wood-rot fungi are known to be saprophytic, they are grown on most of the organic and decaying matters. These fungi have an ability to produce coloured pigments in in-vitro cultures when they are grown in specific growth media. The pigment producing capacity of such fungi depends on the composition and pH of media. Various coloured compounds can be obtained by changing the concentration of component of growth media for such fungi. The most commonly used growth media for in-vitro culturing of woodrot fungi are Potato dextrose agar (PDA) and Potato dextrose broth (PDB) and Sabouraud dextrose agar (SDA) and Sabouraud dextrose broth (SDB). Current study conducted of Ganoderma lucidum, a commonly occurring wood-rot fungi, it has been observed that the production of pigment in G. lucidum was dependent on various growth media and their components. The fungus forms white patchy, non-pigmented colonies when cultured on PDA medium whereas it showed pigmented growth on SDA medium. Further, by replacing each nutrient component in the growth medium one by one, it has been determined that the component plays a vital role in pigment production by G. lucidum. It has been remarkably established that pigment production in the fungus is totally dependent on peptone concentration. Additionally, Nature of pigment has been studied by carbon and hydrogen ratio which has been in correlation with the substrate provided in the growth medium. The pigment obtained by the study were further evaluated for thermal and pH stability which showed promising results as pigment was unaffected till 60°C and there was no colour change detected with various pH solutions. Therefore, this study focuses on the potential applications of pigment obtained from G. lucidum as a natural pigment in textile and food industry by evaluating proper cytotoxic and structural analytical studies in future.

Authors :
Mona Kejariwal : Department of Botany, RD and SH National College and SWA Science College, Bandra west, Mumbai, Maharashtra, India.
Sakshi Soni, Saloni Koli, Eeshrita Jog, Sayok Ghosh, Deepak Singh, Surabhi Parker, Tanvi Patil, Khushboo Chouhan, Akash Kushwaha and Kanika Shetty
Department of Biotechnology, RD and SH National College and SWA Science College, Bandra west, Mumbai, Maharashtra, India.

 

DOI: https://doi.org/10.32381/JPSR.2022.38.01.17