Collection, Isolation, Purification and Pathogenicity of Banded Leaf and Sheath Blight Disease of Maize Caused Rhizoctonia solani f. sp. sasakii

By : Poonam Joshi , S.S. Sharma , Pinki Devi Yadav , Sumit Kumar Yadav

Page No: 133-138

Abstract
Banded Leaf and Sheath Blight (BLSB) disease of Maize Caused by Rhizoctonia solani f. sp. sasakii is one of the most severe disease causing serious yield losses each year. In recent years, Rhizoctonia solani is fitting more prevalent in agricultural areas where climate change is leading to higher temperatures. We conducted a experiment in which, a series of fungal collection, isolation and identification from the infected plant parts. The plant showed typical symptoms like the disease lesion are characterized by the presence of alternate bleached area or zones, which are initially water soaked and narrow purple brown bends oriented perpendicular to long axis of leaves or leaf sheath. The infected isolates may be seen on the affected tissues after reinoculation and produced mycelia growth and sclerotia on both plants and culture plates. On re-isolation it was found that the fungus was identical to the original isolate. It was observed that the infection was much higher in inoculated plants as compared to control.

Authors :
Poonam Yadav, S.S. Sharma, Pinki Devi Yadav and Sumit Kumar Yadav
Department of Plant Pathology, Rajasthan of College of Agriculture, Maharana Pratap University of Agriculture and Technology (MPUAT), Udaipur (Rajasthan), India.
 

DOI: https://doi.org/10.32381/JPSR.2022.38.01.13