In-Vitro Studies and Multiple Shootlet Induction in Cyathocline purpurea (Buch.- Ham. ex D.Don) Kuntze

By : Ruchika Dani , Mahavir Gosavi

Page No: 111-116

Abstract
An efficient micropropagation protocol based on multiple shootlet induction has been standardized in Cyathocline purpurea, a medicinal plant. The nodal and leaf cuttings were cultured on MS medium supplemented with 0.5 mg/mL BAP, 0.5 mg/mL NAA and a combination of 0.5 mg/mL BAP + NAA. Multiple shootlet induction was observed on MS medium supplemented with the combination of 0.5 mg/mL BAP + NAA using nodal explant. The shootlet thus induced, was 2.5 cm in length and had cotyledonary leaves on it. This shootlet was further transferred to another culture vial containing the same media. Phytochemical screening was done to check the presence of phenolics, steroids, alkaloids, glycosides and flavonoids using three different solvents viz., Petroleum Ether, Acetone and Ethanol. Antibacterial activity was seen against E. coli. Antioxidant assay was performed by checking the radical scavenging activity using DPPH.

Authors :
Ruchika Dani : PG student, Department of Botany, S.I.E.S. College of Arts, Science and Commerce at Sion, Mumbai
Mahavir Gosavi : Associate Professor and Head, Department of Botany, S.I.E.S. College of Arts, Science and Commerce (Autonomous), Sion (W), Mumbai.

 

DOI: https://doi.org/10.32381/JPSR.2022.38.01.10