Biosynthesis of Silver Nanoparticles using various botanicals and evaluation of its antimicrobial property

By : Gulab S. Gugale, Bhushan P. Bhusare, Mukund S. Ambawade, Nitin S. Kadam, Akshay B. Shinde

Page No: 371-379

Abstract 
The present study deals with biosynthesis of Silver Nanoparticles using various plant extract and evaluation of its antimicrobial property. Silver Nanoparticles (AgNPs) were biosynthesized using plants extract of Azadirachta indica (Neem), Cymbopogon citratus (Lemon grass), Piper betle (Betele leaf) and Trachyspermum ammi (Carom leaf) separately. Silver nitrate was used as substrate and botanical extract as biotemplate for biological synthesis of AgNPs. It was characterized by UV–vis spectrophotometer, X-ray diffractometer (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). The antimicrobial property of the AgNPs were carried out by well diffusion method using gram positive bacteria, Staphylococcus aureus and gram negative bacteria, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. The highest antimicrobial activity was observed against K. pneumoniae (12±0.26 mm) and E. coli (11±0.11 mm) for AgNPs synthesized using P. betle and C. citratus leaf extracts. The AgNPs synthesized using T. ammi was showed highest antifungal activity against Aspergillus niger. The present study report, the enhanced antimicrobial property of AgNPs synthesized using medicinal plants as reducing and capping agents which would be a promising alternative drug for the treatment of wound infections and skin infections with reduced hazards.

Authors :
Gulab S. Gugale : Department of Chemistry, P.G.K.M’s Haribhai V. Desai College, Pune -411002, Maharashtra, India.
Bhushan P. Bhusare : Department of Life Science (School Of Science), Sandip University Nashik- 422213, Maharashtra, India.
Mukund S. Ambawade : Department of Microbiology, P.G.K.M’s Haribhai V. Desai College, Pune -411002, Maharashtra, India.
Nitin S. Kadam : Central Instrumentation Facility (CIF), Savitribai Phule Pune University, Pune -411007, Maharashtra, India.
Akshay B. Shinde : Department of Biotechnology, Modern College of Agricultural Biotechnology, Paud, Pune- 412108, Maharashtra, India.

 

DOI: https://doi.org/10.32381/JPSR.2021.37.02.18