In Vitro Propagation of Water Caltrop (Trapa Natans.)

By : Imtiyaz Rasool Parrey

Page No: 17-22

Abstract
Present investigation was carried out with the objectives to optimize the conditions of culture medium, shoot proliferation and rooting for mass production of water caltrop trees (Trapa natans.). For sterilization of axillary bud, explants and shoot apexes of wonderful water caltrop treatment, involving HgCl2 (0.1 %) for 3 min gave better sterilization and efficient in vitro propagation is described for wonderful water caltrop using shoot tip and axillary bud explants. The best media for establishment was observed on DKW medium just for the first three weeks. After that, explants sub cultured on WPM medium for proliferation and elongation. ½MS and full MS was used for rooting. Maximum number of shoot (3.9) per explants and shoot length (1.5 to 2.95 cm) were obtained on WPM medium supplemented with 0.8 mg/l B A P + 0.01 mg/L IBA in medium. The highest rooting number was recorded on ½MS medium containing IBA 1mg/l. (1.4) roots per explant. Significantly, higher number of shoots (2.5) and maximum length (2.95cm) of shoot were recorded in 3 percent sucrose, (3000 lux) light intensity and pH 5.8. Rooted plantlets were adopted and transferred to the soil successfully.

Author :
Imtiyaz Rasool Parrey : Department of Chemistry, Government Women College, Anantnag, Jammu and Kashmir.
 

DOI: https://doi.org/10.32381/JPSR.2021.37.01.3