Active Anti-Inflammatory Potency of ?-Sitosterol from Woodfordia floribunda Salisb

By : Pankaj H. Naikwadi, Narendra D. Phatangare, Dhananjay V. Mane

Page No: 691-700

Abstract 
The Woodfordia floribunda Salisb has a very important role in the branch of medicinal chemistry as mentioned in literature. The plant contains a variety of bioactive constituents such as alkaloids, steroids, terpenoids, glycosides, and phenolic compounds, out of these sterols and glycosides show antiinflammatory activity. Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used to ease pain and inflammation. The aim of our present study focuses on the isolation of Gama sitosterol and finding out the anti-inflammatory activity of the same. The compound was isolated by using a Soxhlet apparatus and Column chromatography. Then the crude extract of the plant by performing preliminary phytochemical analysis. From this data, we confirmed that the given plant extract has some phenolic, terpenoids, and steroids. After that, the crude extract was sent for GCMS study, and characterization is done by using Mass, 1 H-NMR, and 13C-NMR techniques. The anti-inflammatory potency of an isolated ?-sitosterol compound is measured by Carrageenan induced paw edema model by applying different dose sizes dependent on the weight of the animal, it is compared with standard diclofenac then some statistics and graphical data are made and it is confirmed that the phytol shows anti-inflammatory activity.

Authors :
Pankaj H. Naikwadi : School of Science and Technology, Yashwantrao Chavan Maharashtra Open University, Nashik. Maharashtra, India.
Narendra D. Phatangare : Department of Chemistry, S. N. Arts, D. J. M. Commerce and B. N. S. Science College, Sangamner, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
Dhananjay V. Mane : School of Science and Technology, Yashwantrao Chavan Maharashtra Open University, Nashik, Maharashtra, India.
 

DOI: https://doi.org/10.32381/JPSR.2022.38.02.23