Isolation, Molecular Characterization and Mycochemical Analysis of Aspergillus quadrilineatus

By : Shaila M. , Nafeesa Begum

Page No: 485-495

Abstract
Secondary metabolites of microbial origin, either as pure compounds or crude extracts, provide opportunities for new drug development because of their chemical diversity. The aim of the present study is to isolate Molecular characterization and extract the bioactive compounds from the fungus of A. quadrilineatus (OL979151) in the rhizospheric soil of sunflower and mustard crop fields in Davanagere region. Dilution plate technique was used to isolate the rhizosphere fungi. The total genomic DNA was extracted from isolate of Aspergillus quadrilineatus based on Cetrimide Tetradecyl Trimethyl Ammonium Bromide (CTAB). Mass production of the fungal mat of A. quadrilineatus was established on potato dextrose broth medium. The dried methanol extract of fungus was subjected to HR-LCMS analysis; Methanol extract of A. quadrilineatus fungus shows that the compounds Amobarbital, Pirbuterol, Bambuterol, Cilazaprilat, Oxotremorine, Aminopyrine and Oryzanol as the major constituents. The compounds exhibit analgesic, anti-inflammatory, and antipyretic properties and various therapeutic uses.

Authors :
Shaila M & Nafeesa Begum : Department of Botany, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India
 

DOI: https://doi.org/10.32381/JPSR.2022.38.02.5