Key Determinants of High Frequency Shoot Regeneration in Sesamum Indicum Cotyledons

By : Poudhi Samadder , Neelakantan Arumugam

Page No: 460-471

Abstract
Despite being the oldest edible oilseed crop, sesame has not reached its potential in biotechnology due to its recalcitrant nature in in vitro conditions, and a reliable shoot regeneration protocol remains elusive. In this study, a high frequency shoot regeneration system applicable to at least 45 different varieties of Sesamum indicum is presented. Combinatorial effect of 6-Benzylaminopurine (BAP) and Indole-3- acetic acid (IAA), ideal sucrose concentration and media of choice were explored, demonstrating that N6 medium augmented with 5 mg/l BAP, 1 mg/l IAA, and 5 mg/l AgNO3, as well as an initial culture on 6 per cent sucrose for two weeks followed by 3 per cent sucrose, significantly improves shoot regeneration. In this medium 73 per cent of the genotypes exhibited shoot regeneration ranging from 31 per cent to 72 per cent, marking the first report of such broad testing in sesame.

Authors
Poudhi Samadder: Senior Research Fellow, Department of Biotechnology, Pondicherry University, Puducherry, India.
Neelakantan Arumugam: Professor, Department of Biotechnology, Pondicherry University, Puducherry, India.
 

DOI: https://doi.org/10.32381/JPSR.2025.41.03.11