Scanning Electron Microscopy Analysis and Antimicrobial activity of Tribulus terrestris L.

By : Seema Mahlawat

Page No: 455-459

Abstract
Dried fruits of T.terrestris was collected from Rohtak region of North India and extracted in various solvents (methanol, ethanol, dichloromethane, ethyl acetate and chloroform) to assess their antimicrobial properties. Agar disk diffusion as well as micro dilution method was employed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Enterobacter aerogenes. Morphological effect on bacterial cells was examined by Scanning Electron Microscopy. Methanol extract exhibited highest inhibition, particularly against B. subtilis at the lowest concentration (0.25 mg/ml). SEM images confirmed rupture of bacterial cells, shrinkage and reduction in cellular integrity. This is the first study using SEM to evaluate the impact of Tribulus terrestris fruit extract on bacterial morphology, validating its potential as antimicrobial agent.

Author
Seema Mahlawat: Assistant Professor, Pt NRS Government College Rohtak, Haryana, India.
 

DOI: https://doi.org/10.32381/JPSR.2025.41.03.10