Phytochemical and HPTLC Fingerprint Analysis for Triterpenes Identification from Simarouba glauca DC 

By : Ashida S. , Sushama Raj R V

Page No: 320-333

Abstract: 
With an emphasis on triterpene chemicals, the current study examined the phytochemical makeup of Simarouba glauca hexane extract column fractions isolated from leaves using High-Performance Thin Layer Chromatography (HPTLC). Phenols, flavonoids, terpenes, steroids, and glycosides were detected by qualitative analysis. Significant phytochemical content was revealed by quantitative analysis as: phenols (565.33±12.85 mgGAE/g), flavonoids (255.3±0 mg QE/g), and terpenes (75.7±1.82mg/mg) in the sample. The definitive detection of beta sitosterol in fractions F3, F4, and F5, with Rf values ranging from 0.161 to 0.167, is one of the major findings. Notably, betulinic acid was not present in any of the fractions that were examined, in contrast to what was initially anticipated. Using thorough confirmation techniques, the study compared fluorescence patterns, band hues, and Rf values to those of reference substances. The study emphasises how crucial it is to use several parameters when utilising HPTLC to analyse a specific molecule. The identification of the phytochemicals sheds light on the possible medical uses of Simarouba glauca DC leaf extracts.
 

Authors:
Ashida S. : Research Scholar (CSIR SRF), Department of Botany, Mahatma Gandhi College, Thiruvananthapuram, Kerala,
Sushama Raj R V: Associate Professor, Department of Botany, VTM NSS College, Thiruvananthapuram, Kerala,
 

DOI: https://doi.org/10.32381/JPSR.2025.41.02.13