HPTLC Fingerprint Profile and Quantification of Ursolic acid in Ocimum kilimandscharicum Guerke leaf

By : Ria P. Mathew, Darshana Patil, Avinash Patil

Page No: 137-147

Abstract
Ursolic acid is a triterpenoid with anti-inflammatory, antioxidative, antiviral and antineoplastic activities. The present study aims to identify and quantify Ursolic acid in Ocimum kilimandscharicum Guerke leaf using HPTLC. Ocimum kilimandscharicum Guerke, commonly known as ‘Kapoori Tulsi’ in Hindi and ‘Camphor Basil’ in English is a traditional medicinal plant belonging to family Lamiaceae. Preliminary Phytochemical analysis indicated presence of various phytoconstituents like flavonoids, alkaloids, glycosides, resins, saponins, steroids, triterpenoids, tannins etc. in the leaves of this plant. This study also establishes certain quality control parameters for Ocimum kilimandscharicum Guerke leaf with the help of HPTLC fingerprint profile of methanolic extract. The chromatographic profile of the extract showed a band corresponding to that of standard Ursolic acid. The Rf value as well as the colour of the band was compared with that of standard Ursolic acid which indicated the presence of Ursolic acid in Ocimum kilimandscharicum Guerke leaf. Ursolic acid was further quantified by using HPTLC and was found to be 158 μg Ursolic acid/g of powdered leaf sample. Thus, Ocimum kilimandscharicum Guerke leaves can be considered as an alternative natural source for Ursolic acid.

Authors :
Ria P. Mathew : Senior Research Fellow, Department of Botany, B. K. Birla College of Arts, Science and Commerce, Kalyan, Maharashtra, India.
Darshana Patil : Associate Professor, Department of Botany, Smt. C.H.M. College, Ulhasnagar, Maharashtra, India.
Avinash Patil : Principal and Head, Department of Botany, B. K. Birla College of Arts, Science and Commerce, Kalyan, Maharashtra, India.
 

DOI: https://doi.org/10.32381/JPSR.2025.41.01.14