Pharmacological Studies on Some Medicinal Plants for Bone Disorder

By : Varuna, Devendra Pratap Singh, Alok Tripathi, Divya Singh, Rajiv Gupta

Page No: 75-86

Abstract
The study presents a detailed analysis of the fingerprint profiling of ethanolic extracts derived from two well-known herbal plants, Curcuma longa and Moringa oleifera, using Soxhlet extraction. The extracts were comprehensively analyzed through Thin-Layer Chromatography (TLC) and High- Performance Liquid Chromatography (HPLC). These analytical techniques revealed distinct fingerprint profiles, identifying bioactive compounds such as curcuminoids and quercetin. To evaluate the osteoprotective effects of these extracts, an experimental study was conducted using a fracture model in Balb/c mice. The mice, subjected to a drill hole injury in the femur metaphysis, were treated with oral doses of the ethanolic extracts. The treated groups demonstrated enhanced fracture healing compared to the control group. This was evidenced by increased callus formation observed through micro-CT imaging, improved histological features indicative of accelerated bone regeneration, and superior biomechanical strength as confirmed by biomechanical testing. The findings suggest that ethanolic extracts of C. longa and M. oleifera may serve as a natural therapeutic strategy to promote fracture healing and prevent osteoporotic conditions. The distinct chemical profiles identified through TLC and HPLC analyses support their potential efficacy. However, further research is necessary to elucidate the underlying mechanisms and optimize dosage regimens for clinical applications.

Authors :
Varuna : Research Scholar, Department of Pharmaceutical Chemistry, School of Pharmacy, BBD University, Ayodhya Road, Lucknow, Uttar Pradesh, India.
Devendra Pratap Singh : Research Scholar, Division of Endocrinology, Central Drug Research Institute (C.D.R.I.- C.S.I.R.), Jankipuram, Lucknow, Uttar Pradesh, India.
Alok Tripathi : Research Scholar, Division of Endocrinology, Central Drug Research Institute (C.D.R.I.- C.S.I.R.), Jankipuram, Lucknow, Uttar Pradesh, India.
Divya Singh : Senior Principal Scientist and Professor (AcSIR), Division of Endocrinology, Central Drug Research Institute (C.D.R.I.-C.S.I.R.), Jankipuram, Lucknow, Uttar Pradesh, India.
Rajiv Gupta : Principal & Dean, Department of Pharmacognosy, School of Pharmacy, BBD University, Ayodhya Road, Lucknow, Uttar Pradesh, India.
 

DOI: https://doi.org/10.32381/JPSR.2025.41.01.8