Antibacterial and Antibiofilm Activity of Methanolic Leaf Extract of Sphenodesme involucrata var. paniculata (C.B.Clarke) Munir against Streptococcus pyogenes

By : Athira S., Sreeranjini S.

Page No: 54-60

Abstract
Streptococcus pyogenes is a Gram positive bacterium that causes infectious diseases like rheumatic fever, Rheumatic Heart Disease (RHD) etc. which can be prevented only by long term penicillin usage. Emergence of antibiotic resistant bacteria and side effects of long term usage of antibiotics are posing a serious threat to mankind. Plant based alternatives are considered as an efficient remedy for this. Current study was aimed to evaluate the antibacterial and antibiofilm activity of the leaf methanolic extract of Sphenodesme involucrata var. paniculata (C.B.Clarke) Munir, an ethno medicinal plant against Streptococcus pyogenes. The antibacterial activity was analysed by agar well diffusion assay and antibiofilm activity by in vitro anti-biofilm assay. The extract showed a better inhibition zone at 100 μL and showed a potent anti-biofilm activity in a dose dependent manner with an inhibition percentage of 97.1 at 1000 μL concentration. The results suggest that the usage of this plant can be considered as a defence against S. pyogenes with further in vivo analysis.

Authors :
Athira S. : Research Scholar, Department of Post Graduate Studies and Research in Botany, Sanatana Dharma College, Alappuzha, Kerala, India.
Sreeranjini S. : Assistant Professor, Department of Post Graduate Studies and Research in Botany, Sanatana Dharma College, Alappuzha, Kerala, India.
 

DOI: https://doi.org/10.32381/JPSR.2025.41.01.6