Phytochemical Screening and Quantitative Analysis of Total Alkaloid, Phenolic, Flavonoid and Tannin Content Using Spectrophotometric Assays in Trichosanthes bracteata Lam.

By : Mayuresh J. Patil, Sangeeta A. Godbole

Page No: 317-325

Abstract
Trichosanthes bracteata, belonging to the family Cucurbitaceae is a rare wild plant and widely distributed in India. This plant has been reported to be used by tribals due to its excellent ethnobotanical medicinal uses. This paper presents the phytochemical content, qualitative and quantitative analysis of T. bracteata. Various parts of this plant were analyzed qualitatively to evaluate the presence of secondary metabolites in both polar and non-polar solvents. Phenolics, flavonoids, alkaloids and tannins, were the most abundant classes of compounds in methanolic extracts of both leaf and flower samples. Flavonoids were also detected in the leaf petroleum ether extracts. Terpenoids (Diterpenes and phytosterols) were strongly detected in stem (methanolic) as well as root and seed extracts of both solvents. Spectroscopic methods of estimation were further used to quantify the total phenolics, flavonoids, alkaloids and tannin content in methanolic extracts of various plant parts. Leaf extracts confirmed the presence of the highest amounts of flavonoids (8.14 mg/ gm), followed by alkaloids (1.164 mg/gm) and phenolics (1.109 mg/gm). The flower extracts confirmed the highest amounts of total phenolic compounds (9.079 mg/gm) and tannins (0.954 mg/gm).

Authors:
Mayuresh J. Patil : Research Scholar, Department of Botany, Jai Hind College, Maharashtra, India.
Sangeeta A. Godbole : Associate professor, Head and Research Guide, Department of Botany, Jai Hind College, Maharashtra, India.
 

DOI: https://doi.org/10.32381/JPSR.2024.40.02.12