In-vitro Antioxidant and Anti-inflammatory Properties in methanol leaf extract of Calanthe masuca (D. Done). Lindl. (Orchidaceae) of terrestrial an endangered orchid of Kolli Hills, in Tamil Nadu

By : Duraisamy Kavitha, Veeraiyan Nandagopalan, Seedar Deborah

Page No: 154-162

Abstract
In the present study, we assessed the antioxidant and anti-inflammatory activities of the methanol leaf extract of Calanthe masuca (D. Done). Lindl through in-vitro methods. Antioxidant activity has been investigated using various assays, including 1,1-Diphenyl-2-Picrylthydrezyl (DPPH), hydrogen peroxide, ABT-assay, and nitric oxide radical scavenging activity, across different concentrations (500, 250, 100, 50, and 10 μg/ml). The in-vitro anti-inflammatory activity was evaluated through the albumin denaturation assay, measuring inhibitory activity at concentrations of 500, 250, 100, 50, and 10 μg/ml. A standard drug employed for comparison in the anti-inflammatory study. The results indicated significant antioxidant activity in the methanol leaf extract, as evidenced by IC50 values of 97.35 µg/ml (DPPH), 79.24 µg/ml (ABT-assay), 51.07 µg/ml (Hydrogen peroxide) and 28.62 µg/mg (Nitric oxide radical scavenging). The in-vitro anti-inflammatory assessment revealed inhibition of heat-induced albumin denaturation, with an IC50 value of 76.03 µg/mg. To conclude, Calanthe masuca (D. Done). Lindl’s methanol leaf extract has shown promising pharmaceutical and pharmacological activities, which highlight its potential therapeutic significance.

Authors:
Duraisamy Kavitha : PG and Research, Department of Botany, National College, Affiliates to Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Veeraiyan Nandagopalan : Associate Professor, Department of Botany, National College, Affiliated to Bharathidasan Univeristy, Tiruchirappalli, Tamil Nadu, India.
Seedar Deborah : Assistant Professor, Department of Biotechnology, Jamal Mohammed College, Affiliated to Bharathidasan College, Tiruchirappalli, Tamil Nadu, India.
 

DOI: https://doi.org/10.32381/JPSR.2024.40.01.18