In vitro Propagation of a Dendrobium Hybrid (D. Apichart Rainbow x D. bigibbum var. compactum): An Important Orchid used for Potted Flowers

By : Johnnita Tirkey , Nihar Ranjan Nayak

Page No: 70-78

Abstract
Dendrobium hybrid orchids are widely used for potted flowers as well as for cut flowers. In this study, the Dendrobium hybrid (D. Apichart Rainbow x D. bigibbum var. compactum) widely used as the potted f lower because of the unique flower qualities was used. Availability of the planting materials is the major constraint for which the use of tissue culture technology is highly essential. Seed pods were formed through hand pollination on the plants growing at the orchidarium and mature seeds were brought to the laboratory. After surface sterilization and culturing on Murashige and Skoog’s (1962) medium for germination and further seedling development. Effects of different plant growth regulators on the seed germination were thoroughly analysed, and it was found that the seeds on BAP 0.25 mg/l showed the highest rates (90. 52%) of germination at 90 days of culture. On the same medium, at 18 months of culture healthy seedlings were formed, each had 2.0 roots and 3.0 leaves. The seedlings reached a height of 4cm at 18 months of culture. The young shoots produced on BAP 0.5 mg/l were used as the explants for the production of new shoots. The inclusion of BAP 2.5 mg/l showed the highest production of 15.0 shoots from each explant. The new shoots were transferred to MS nutrient medium containing 2.0 mg/l IBA for efficient root induction in which each shoot produced 4.33 numbers of roots at 180 days of culture. In vitro, raised shoots were successfully acclimated in the greenhouse for further growth and development.
 

Authors:
Johnnita Tirkey : Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India.
Nihar Ranjan Nayak : Regional Plant Resource Centre, Nayapalli, Bhubaneswar, Odisha, India.
 

DOI: https://doi.org/10.32381/JPSR.2024.40.01.9