Functional Characterization of OsbHLH185 Transcription Factor in Arabidopsis Under Abiotic Stresses

By : Roshan K. V. Remesh , Jyothish Madambikattil Sasi , Biju Dharmapalan , Dhanya D.S

Page No: 42-49

Abstract
Basic helix-loop-helix (bHLH) transcription factors play a pivotal role in governing various cellular processes associated with growth, development, and stress responses in plants. The current investigation aimed to conduct a functional characterization of a rice bHLH transcription factor gene, OsbHLH185, under several abiotic stress conditions, namely salt stress, mannitol stress, cold stress, and the application of abscisic acid (ABA). Transgenic Arabidopsis lines of OsbHLH185 were produced by over expression techniques and afterward employed for the investigation of stress response. The phenotypic, morphometric, and physiological parameters of the over-expression lines were assessed in the context of abiotic stressors. The transgenic plants with over-expression lines demonstrated a notable increase in tolerance to abiotic stress in comparison to the non-transgenic wild-type plants. The OsbHLH185 over expression lines had increased levels of the ABA biosynthesis gene NCED3 and signaling genes, which may be the cause of the phenotype of abiotic stress tolerance. The findings of this initial study can serve as a valuable resource for future investigations, while the utilization of OsbHLH185 in breeding research holds potential for the enhancement of abiotic stress tolerance in crop species.
 

Authors:
Roshan K. V. Remesh : Department of Biotechnology, Sree Narayana Arts & Science College, Kumarakom, Kerala, India.
Jyothish Madambikattil Sasi : CSIR-National Institute of Science Communication and Policy Research (NIScPR), 14, Satsang Vihar Marg, New Delhi, India.
Biju Dharmapalan : School of Natural Sciences and Engineering, National Institute of Advanced Studies, Indian Institute of Science Campus, Bangalore, Karnataka, India.
Dhanya D. S. : Department of Botany, Sree Narayana College for Women, Kollam, Kerala, India.
 

DOI: https://doi.org/10.32381/JPSR.2024.40.01.6