Phytochemical Analysis of Rubus fockei Utilized in the Ethnomedicinal Traditions of the Kodagu District and its Cytotoxicity Evaluation on Hep G2 Cell Line

By : Ashitha Ganesh B., M. P. Raghavendra, A. G. Devi Prasad

Page No: 15-25

Abstract
Among the numerous medicinal plant species growing in the wild, vaale pann or taxonomically the Rubus fockei sp. occupies its unique place in the traditional knowledge of Kodagu district in Western Ghats. In the present study, phytochemical screening of methanolic, and aqueous extracts of the leaves has revealed the presence of 12 different phytocompounds. Anticancer activity of both the extracts revealed significant inhibitory activity against HepG2 cell lines. HPLC analysis of the aqueous and methanolic extracts gave peaks at retention times 15.47, 34.44 and 21.541, 34.382, 34.677 respectively. LC-MS spectra of extracts of Rubus fockei from aqueous extract showed the presence of six compounds in the positive ion mode and nine compounds in the negative ion mode while the methanol extract showed the presence of 19 compounds in positive ion mode and 24 compounds in the negative ion mode. FTIR analysis of methanolic and aqueous extract revealed the presence of about 17 functional groups. The present study showed that extracts of Rubus fockei leaves possess anticancer properties, which could be explored further for pharmaceutical applications.

Authors:
Ashitha Ganesh B. : Department of Studies in Environmental Science, University of Mysore, Mysuru, Karnataka, India.
M. P. Raghavendra : Postgraduate Department of Microbiology, Maharani’s Science College for Women Mysuru, Karnataka, India.
A. G. Devi Prasad : Department of Studies in Environmental Science, University of Mysore, Mysuru, Karnataka, India.
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DOI: https://doi.org/10.32381/JPSR.2024.40.01.3