Anticancer Property of Alstonia scholaris Linn Leaf Extract in MCF -7 and MDAMB -231 Breast Cancer Cell Line

By : Dhanakodi Shanmugapriya, Gopalan Jayanthi, Ranga Anantha Sayanam Rajeswari

Page No: 107-117

Abstract
Breast cancer is the major risk factor for mortality globally. Medicinal plant extracts were investigated as a potential source for breast cancer therapy. The current work aims to assess the intracellular ROS production and apoptosis induction by Alstonia scholaris Linn (A. scholaris) leaf extract in breast cancer cell lines. Column chromatography was used to purify bioactive components from an A. scholaris Linn methanolic extract. Fraction with a significant concentration of phytochemicals was utilised for further investigation. The results of DCFH-DA staining revealed a substantial rise in intracellular ROS levels in A. scholaris Linn-treated cell lines. The dual staining technique (AO/EtBr) was used to examine apoptotic changes. Treatment with A. scholaris Linn substantially enhanced the rate of apoptosis in breast cancer cell lines. Furthermore, enhanced p53 gene expression, downregulation of Bcl-2 and BRAC1 genes, and reduced TNF-a and IFN-l concentrations in A. scholaris Linn treated cancer cells demonstrated its anticancer activity.

Authors :
Dhanakodi Shanmugapriya and Gopalan Jayanthi : Assistant Professor, Department of Biochemistry, Government Arts College for Women, Krishnagiri, Tamil Nadu, India
Ranga Anantha Sayanam Rajeswari : Department of Biochemistry, School of Allied Health Sciences, Vinayaka Mission’s Kirupananda Variyar Medical College and Hospitals Campus, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem, Tamil Nadu, India.
 

DOI: https://doi.org/10.32381/JPSR.2023.39.03.11