Starch-based on Electrospun Nanofibrous Scaffold Incorporated with Bioactive Compound from Mollugo nudicaulis for Wound Healing Application

By : Mani Panagal, Siva Shankar Raj

Page No: 31-41

Abstract
The aim of this research was to develop nanofibrous scaffolds using starch and incorporating 12-(10-carboxydecanoyloxy)-12-oxododecanoic acid (Compound-1) from the ethanolic extract of Mollugo nudicaulis. Compound-1 was added to starch solutions at different concentrations (0, 20, 30, and 40% v/v) and transformed into nanofibers through electrospinning. The resulting electrospun fibers were analyzed for their morphology, size distribution, thermal stability, FT-IR spectra and wound healing potential. The nanofibers loaded with compound-1 exhibited a uniform morphology and had average diameters ranging from 76 to 98 nm. Moreover, the compound-1 encapsulated within the nanofibers demonstrated higher thermal stability compared to the free compound-1. FT-IR analysis revealed interactions between the starch and compound-1. Notably, the nanofibers loaded with 40% compound-1 exhibited significantly enhanced viability in HaCat cells. Western blot analysis showed increased expression levels of VEGF and PDGF proteins, suggesting that the developed nanofibers could promote angiogenesis and cell proliferation, thereby potentially accelerating the wound healing process. Based on these results, it can be concluded that the developed starch-based electrospun nanofibrous scaffolds, incorporating compound-1 from Mollugo nudicaulis, possess potent wound healing properties by activating the VEGF and PDGF signaling pathways. Nevertheless, further in vitro and in vivo studies are necessary to validate these findings.

Authors :
Mani Panagal and Siva Shankar Raj : Department of Biotechnology, Annai College of Arts and Science (Affiliated by Bharathidasan University), Kovilacheri, Kumbakonam, Tamil Nadu, India
 

DOI: https://doi.org/10.32381/JPSR.2023.39.03.4