In Vitro Studies of Curcuma Longa L. and Assessment of Genetic Uniformity through Chemo and DNA Profiling

By : Sachin S. Kharade, Kailash Ch. Samal, Gyanalok Das, G.R. Rout

Page No: 213-219

Abstract
Curcuma longa is an important medicinal plant species having high curcuminoids. The present investigation illustrates the in vitro shoot multiplication, genetic and chemo-profiling assessment of regenerated plants by using DNA and HPTLC analysis. The shoot buds were used for direct shoot induction and regeneration by using MS medium supplemented with 1 – 5 mg/l 6-benzylaminopurine (BAP) alone or in combination with 1.0 – 2.0 mg/l 1-napthaleneactic acid (NAA). Shoot multiplication was observed in MS medium fortified with 2 - 3 mg/l BAP. Combination of 2.5 mg/l BAP and 1.5 mg/ l NAA resulted the highest number of shoot multiplication. About 8.2 shoots/culture was observed in MS medium supplemented with 2.5 – 3.0 mg/l BAP and 0.5 mg/l NAA within 4 weeks of culture. In vitro root induction was achieved in MS medium supplemented with 0.5 mg/l IBA or NAA within 2 – 3 weeks of culture. Healthy regenerated plantlets were acclimatized in the sterile mixture of garden soil, compost and cow dung in the ratio of 1:1:1 with 90% survivality. DNA profiling were conducted to study the genetic stability of the regenerated plantlets. The curcumin, demethoxycurcumin (DMC) and bis-demethoxycurcumin (BDMC) content in in vitro raised plantlets were estimated by using HPTLC fingerprint.

Authors :
Sachin S. Kharade, Kailash Ch. Samal and G.R. Rout : Dept. of Agricultural Biotechnology, College of Agriculture
Gyanalok Das : AICRP on Medicinal and Aromatic Plants, Orissa University of Agriculture and Technology, Bhubaneswar- 751003, Odisha