Isolation of genomic DNA from mature leaves and spikes of black pepper (Piper nigrum L.)

By : Ashish Subba, Swapna Alex, Soni K. B, Deepa S. Nair, Reghunath B. R

Page No: 21-25

Abstract
Obtaining intact genomic DNA of sufficiently high quality that is amplifiable using PCR from black pepper is a challenging task, as it is rich in polyphenolic compounds. Methods that are currently available recommend the use of young leaves which may not be available in all seasons. In this paper, a modified protocol of the CTAB method for the extraction of genomic DNA from mature leaves and spikes of black pepper is presented. This method employs higher concentrations of CTAB, NaCl, EDTA and PVP. Good yield of DNA ranging from 400 to 500 ng/?l with A260/280 ratio of 1.7 to 2.0 was obtained. The isolated DNA was successfully amplified by RAPD primers.

Authors :
Ashish Subba, Swapna Alex, Soni K. B., Deepa S. Nair and Reghunath B. R. : Department of Plant Biotechnology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, India, Pin- 695 522