Effect of Plant Growth Regulators on in Vitro Cultures of Fennel (Foeniculum Vulgare Mill.) Genotypes

By : R P Didel, Ganga Ram, Santosh Choudhary, M. L. Jakhar

Page No: 17-19

Abstract
Healthy and contamination free in vitro fennel shoot apex explants of five genotypes were used in order to study the effect of plant growth regulators on shoot multiplication, root induction and biomass accumulation on MS medium. The cultures were incubated in a culture room at temperature of 25 + 2°C with relative humidity of 55 + 5 percent were exposed to approximately 3000 lux light intensity for fourteen hours per day, significantly highest shoot multiplication ratio and biomass accumulation were observed in fennel genotype GF-1 at 1.0 mg/l BAP followed by local fennel. The explants treated with 1.0 mg/l NAA induced highest number of roots in RF-101, however, treatment of BAP was insufficient to induce roots at all. Auxins induces more number of roots when supplemented alone in the medium.

Authors :
R P Didel, Santosh Choudhary, M L Jakhar and Ganga Ram : Department of Plant Breeding and Genetics, SKN College of Agriculture, (Sri Karan Narendra Agriculture University), Jobner – 303329. India