Genotypic Differences for Callus Induction and Plantlet Regeneration in Coriander (Coriandrum Sativum L.)

By : Santosh Choudhary, R P Didel, M. L. Jakhar, Ganga Ram

Page No: 13-16

Abstract
Shoot apex and hypocotyl explants of seven coriander (Coriandrum sativum L.) genotypes were cultured on MS medium supplemented with varying concentrations of cytokinins and auxins. Profuse callus induction was observed in hypocotyls explants at 1.0mg /l 2,4-D and this callus remained potent for longer time. Shoot apex derived BAP (1.0 mg/l) callus was also subcultured on different concentrations of plant growth regulators. However, it did not regenerate in complete plant even after various manipulations. Shoot morphogenesis was observed in hypocotyls derived callus cultures upon subculture on 0.5 mg/l BAP. Genotype RCr-20 induced maximum de novo shoots from callus followed by RCr-446. De novo developed shoots from callus grown on the medium containing 0.5 mg/l NAA induced roots at the base and complete plants were obtained.

Authors :
Santosh Choudhary, R P Didel, M L Jakhar and Ganga Ram : Department of Plant Breeding and Genetics, SKN College of Agriculture, (Sri Karan Narendra Agriculture University), Jobner – 303329. India