Efficiency of Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) Markers for Molecular Characterization of Eruca sativa Varieties

By : Jyoti Ushahra , C P Malik

Page No: 75-82

Abstract
Genetic diversity evaluations among six Eruca sativa (Miller) Thell. varieties were determined using RAPD and ISSR markers.Total genomic DNA was extracted by CTAB method given by Doyle and Doyle and 65 RAPD and 51 ISSR primers were used. Out of 65 tested primers, 27 primers were amplified, of which 13 were polymorphic and 14 were monomorphic. The number of amplicons per primer ranged from 5 to 11. The size of the bands ranged from 250 bp to 1900 bp. The polymorphic primers amplified 93 bands of which 24 were polymorphic. The polymorphism was maximum (44.4 %) with OPA 6 primer and was low (12.5 %) with OPA 15. Similarly, of the 51 tested primers, 36 primers gave amplification products. Out of 36 primers, 15 primers gave polymorphic bands while 20 primers were monomorphic. The number of amplicons per primer ranged from 5 to 14. The size of the bands ranged from 200 bp to 1800 bp. The polymorphic primers amplified 148 bands of which 21 were polymorphic. The polymorphism was maximum (33.3 %) with primers UBC 835, UBC 858 and was low (7.1 %) with UBC 864. The purpose of present study was to characterize Eruca sativa varieties at molecular level and valuable information could be successfully utilized to determine genetic diversity and genetic relationship of various varieties.

Authors :
Jyoti Ushahra and C.P. Malik : School of Life Sciences, Jaipur National University, Jaipur-302 017, Rajasthan.