In Vitro Regeneration Studies In Wild Crucifers

By : Javeed A. Lone , Dr. S. K. Gupta , Shabir H. Wani , Rayees A. Lone , Mamta Sharma , M. N. Khan

Page No: 129-134

Abstract
“In vitro Regeneration Studies in wild crucifers” was carried out in the Biotechnology Laboratory, Division of Plant Breeding and Genetics. Two wild crucifers were cultured on MS Media supplemented with growth regulators at different concentrations. The highest per cent of aseptic seeds and survival of seedlings was observed after one and four weeks when treated with HgCl2 (0.1%) for 3 minutes. Moderate response was shown at 2, 4-D 2.0 and 2.5 mg/l by B. tournefortii and B. fruticlusa. No response was observed in the MS media supplemented with different concentrations of NAA. The different concentrations of media showed a significant difference in the callus induction frequency (CIF) and regeneration frequency (RF) for the genotype tested. The organic adjuvant (coconut water) was used to increase the callus induction and shoot regeneration efficiency. Thus it is concluded that 2, 4-D 2.0 mg/l and 2.5 mg/l should be applied for maximum callus induction and BAP (5.0 mg/l) + 2, 4-D (0.5 mg/l) for shoot regeneration.

Authors :
Javeed A. Lone and S. K. Gupta : Sher-e- Kashmir University of Agricultural Science and Technology Chatha-190008 Jammu, Division of Genetics and Plant Breeding
M. N. Khan, Shabir H. Wani, Rayees A. Lone and Mamta Sharma :Sher-e- Kashmir University of Agricultural Science and Technology Shalimar-190025 Kashmir, Division of Genetics and Plant Breeding