Callus regeneration of Shami (Prosopis cineraria) an Endangered Plant of Braj Region of Uttar Pradesh

By : Divya Pathak, Rajneesh K. Agnihotri, Rajendra Sharma

Page No: 95-100

Abstract
India has ranked first in the term of flora in rich biodiversity which contain medicinal economical and high value plants. Prosopis cineraria considered as an auspicious plant belongs to family Fabaceae under the endangered category. So the conservation of this plant is very important. Through in-vitro technique effort has been made to conserve and protect is important plant species. Callus culture was initiated from explants on the Murashige and Skoog medium having 30 g/l sucrose, 0.8% agar supplemented with various concentrations of BAP + NAA (2+1, 4+1, 5+1 mg/l). The maximum induction (62.50%) was observed in 2 mg/l BAP + 1 mg/l NAA under and above the surface of medium. At the beginning explants produced whitish yellow calli after 4 weeks of inoculation. In 3 mg/l BAP and in 2 mg/l BAP + 1 mg/l NAA maximum callus were developed. BAP (2 mg/l) and NAA (1 mg/l) showed the good performance and calli were formed in highest number after 12 weeks of subculture.

Authors :
Divya Pathak, Rajneesh K. Agnihotri and Rajendra Sharma : Department of Botany, S.L.S. Khandari Campus, Dr. B.R. Ambedkar University, Agra