Molecular Characterization of Fennel (Foeniculum vulgare Mill.) accessions using Start Codon Targeted (SCoT) markers

By : Chitrangda Yadav, C P Malik

Page No: 37-44

Abstract
Fourteen accessions collected from different locations in Northern India. Seeds of defined varieties grown in Rajasthan were procured from the Department of Plant Breeding and Genetics, S.K.N College of Agriculture, Jobner, Jaipur (Rajasthan) India were studied using Start Codon Targeted (SCoT) markers. Total thirty-six SCoT primers were screened to study the diversity, out of which thirty five primers could amplify reproducible bands. A part of that SCoT 20 primer could not amplify reproducible bands. These primers were selected for molecular characterization of fourteen Foeniculum vulgare accessions, which produced a total of 256 bands (7.0 bands per primer), of which 240 were polymorphic. The analysis revealed 85.69% polymorphism for SCoT, respectively. Similarity coefficient was used to detect the phylogenetic relationship, subsequently dendogram was constructed for SCoT data. The UPGMA cluster analysis revealed two distinct sub-clusters in the dendogram. In cluster1 thirteen accessions were grouping together whereas variety 6 present in cluster 2. Accession number 6 was different to all thirteen. The genetic diversity explained by gene targeted markers shows that SCoT marker can be very effective for characterization of genetic diversity in medicinal plant like Foeniculum vulgare.

Authors :
Chitrangda Yadav : PhD Research Scholar, School of Life Sciences, Jaipur National University, Jaipur302017, Rajasthan, India
C.P.Malik : Advisor (Academics), School of Life Sciences, Jaipur National University, Jaipur302017, Rajasthan, India.