Genetic Diversity Assessment in Trachyspermum ammi L. Sprague Using CDDP and CBDP Markers

By : Priya Tomar, C P Malik

Page No: 27-36

Abstract
Trachyspermum ammi.L. Sprague commonly known as ‘Ajwain’ belonging to family Apiaceae is distributed throughout India and is mostly cultivated in Gujarat and Rajasthan which constitutes various phytochemical constituents and proven to possess various pharmacological activities. Genetic diversity study was carried out in twelve accessions of Trachyspermum ammi by using Conserved DNA-Derived Polymorphism (CDDP) and CAAT Box-Derived Polymorphism (CBDP) markers. Total genomic DNA was extracted from young leaves following the standard CTAB method. A total of 21 CDDP primers and 25 CBDP primers were used for the analysis. In CDDP, method uses single 15-19 primers for PCR at annealing temperature of 500 C while CBDP primers are 18 nucleotides long but with same annealing temperature 500 C. The present investigation revealed that these novel molecular markers could be successfully utilized to determine genetic diversity and genetic relationship of various accessions of Trachyspermum ammi.

Authors :
Priya Tomar : PhD Scholar, School of Life Sciences, Jaipur National University, Jaipur 302017 Rajasthan, India
C.P. Malik : Academic Advisor, School of Life Sciences, Jaipur National University, Jaipur 302017 Rajasthan, India