Study on Genetic Diversity of some Morpho types of Verbesina encelioides (Cav.) Benth. & Hook. f.ex A. Gray using random amplified polymorphic DNA (RAPD) and inter sequence repeat (ISSR)

By : Damini Singh , C P Malik

Page No: 63-69

Abstract
Present investigations were carried out in Verbesina encelioides. Morphological studies were carried out using multivariate data analysis method. Dissimilarity coefficient value ranged from 1.41 to 3.62 percent in all ten morphotypes were recognised from field studies. Morphotype 2 and 8 were found entirely similar to each other as revealed from clustering based on euclidian distances. The two markers, RAPD and ISSR used proved as the effective tools to evaluate genetic diversity in different morphotypes of V. encelioides selected. The dendrogram constructed based on Euclidian distances from the morphological data revealed that the 10 genotypes were divisible into two clusters; the first cluster included only morphotype 1 remaining were included in second cluster. Cluster II was subdivided into two sub clusters i.e. I (a) 2, 8, 5, 6, 10 and 9 and I(b) 3, 4 and 7. Each morphotype is shown to have unique DNA fingerprint and could be used to identify individual morphotype.

Authors :
Damini Singh and C P Malik : School of Life Sciences, Jaipur National University, Jaipur