Standardization of Micropropagation in Gliricidia [Gliricidia sepium (Jacq.) Steud.]

By : Kaushlya Choudhary, M.L. Jakhar, Aparna

Page No: 11-15

Abstract
An efficient micropropagation protocol has been developed from soft nodal stem segment explant of Gliricidia [Gliricidia sepium(Jacq.) Steud.]. The present investigation was carried out with the objectives of optimization of rapid micropropagation protocol, influence of antioxidants on micropropagation and influence of photoperiod on micropropagation of Gliricidia sepium. Explants (Soft nodal stem segments) were inoculated on MS medium containing varying concentrations of cytokinins and auxins either singly or in combinations. The cultures were incubated at 25 ± 2 0 C under 14: 10 hours photoperiod with a light intensity of 3000 – 3500 lux. Maximum number of shoot bud induction was obtained when MS medium is supplemented with 0.5 mg/l BAP with 100 per cent frequency. MS medium containing 2.0 and 2.5 mg/l NAA resulted in profuse callusing. 2.0 mg/l NAA showed highest number of roots/explant but the roots were thick. Addition of 250 mg/l ascorbic acid induced maximum number of shoot buds (5.1) in comparison to other antioxidants. 0.5 mg/l IAA exhibited long and thin roots when supplemented in MS medium. Among all photoperiod regimes, 14: 10 was found to be most effective for shoot bud induction at most responsive level.

Authors :
Aparna, M. L. Jakhar and Kaushlya Choudhary : Department of Plant Breeding and Genetics, Sri Karan Narendra Agriculture University, SKN College of Agriculture, Jobner, Jaipur-303329