Effect of Antioxidants on in vitro culture of Pomegranate cv. Sindhuri

By : Ravi Kumar, Anita Burdak, M.L. Jakhar, Komal Sekhawat, Swarnlata Kumawat

Page No: 311-318

Abstract
Present investigation was carried out with the objectives to optimize the effect of antioxidants on shoot proliferation for mass multiplication in different explants like nodal segment and shoot apex pomegranate (Punica granatum L.) cv. Sindhuri. Different explants viz., nodal segment and shoot apex obtained aseptically were inoculated on MS medium supplemented with responsive level (Nodal segment, 2.0 mg/l BAP and shoot apex, 2.5 mg/l BAP) of plant growth regulators for shoot multiplication. Antioxidants viz: activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation. The cultures were incubated at 25±2°C under 14:10 hour’s photoperiod with a light intensity of 3000 lux. Activated charcoal (200 mg/l) was found best antioxidant for maximum shoot bud induction in nodal segment and shoot apex explant through controlling of phenolic compounds.

Authors :
Ravi Kumar, Komal Sekhawat, Swarnlata Kumawat and Anita Burdak : Research scholar, Department of Plant Breeding and Genetics, SKN College of Agriculture, Jobner
M.L. Jakhar : Professor, Department of Plant Breeding and Genetics, SKN College of Agriculture, Jobner