RAPD Analysis of Mustard (Brassica juncea L.) Genotypes Differing in Response to Water Stress

By : Vijeta Sharma, , Pushpa Seth , Sunil K. Khandelwal , Arunabh Joshi , Devendra Jain , Deepak Rajpurohit

Page No: 107-113

Abstract
PCR- based random amplified polymorphic DNA (RAPD) markers were employed to search genetic diversity and association with drought tolerance in fifteen mustard genotypes. Ten morphological observations were recorded and these morphological characters were analyzed using ward’s clustering method to compare with RAPD analysis. Identified the genotypes tolerant to water stress involved growing fifteen genotypes at seedling stage under drought conditions (-5 bar) exerted by PEG-6000. These tests confirmed the seven genotypes were tolerant to water stress. Fifteen out of ten random primers screened revealed polymorphism among the genotypes. A total of 521 amplified fragments were formed by 10 primers and 44 amplicon were obtained. Out of which 26 bands were polymorphic and the level of polymorphism is 56.25 per cent. The average number of polymorphic bands per primer is 2.6. Ten decamer RAPD primers were used for fingerprinting of the genotypes of which primers P10 (S-36) and P5 (OPB-04) produced highest total per cent match (66.67%) to the drought tolerant genotypes at base pair 400 and a 820 respectively. Based on polymorphism four RAPD primers S-36, S-30, OPC-09 and OPC-05 were found to be highly discriminative. The result showed that there is a relationship among morphological characters, molecular analysis and stress genotypes.

Authors : 
Vijeta Sharma, Pushpa Seth, Sunil K. Khandelwal, Arunabh Joshi, Devendra Jain and Deepak Rajpurohit : Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture & Technology, Udaipur (Rajasthan) 313 001