Genetic Variation among Fennel (Foeniculum vulgare Mill.) Varieties on the basis of Essential Oil Composition and Molecular Markers (ISSR, SCoT, CDDP and CBDP)

By : Chitrangda Yadav , C P Malik

Page No: 45-50

Abstract
Fennel (Foeniculum vulgare Mill.) benefits from a lot of useful medicinal properties. Genetic variance and heritability estimates of traits in a plant’s primary germplasms are needed before planning of a new breeding program. In the present study the genetic diversity for 14 fennel ecotypes were assessed by using molecular markers (ISSR, SCoT, CDDP and CBDP). Across the cultivars, a total of 102 fragments got amplified, of which 692 were polymorphic. Average polymorphic band per primer was 6.7. The highest amounts of polymorphism were observed in variety RF-145 and RF-205.As per diversity analysis; the polymorphic varieties were screened further for essential oil content. Essential oil content was determined by GC-MS. Both varieties were evaluated for their essential oil content and its composition. Essential oil content ranged from 20.05 to 25.21%. Twenty three major compounds including Limonene, ?-ocimene, ? -ocimene, Dihydrotagetone, Tagetone, Cis- Tagetone, TransTagetone. ? -terpinene, Camphene, Sabinine, Mycrene, ? -phyellandrene, ? -terpinene, Linalool, Fenchone, Methyl chavicol, Trans-anethole, Cis-anethole, Camphor, Estragole, ?-elemene, ?-himachalene and ?-himachalene were detected in variety RF-145 and Seventeen major compounds including ?-pinene, Camphene, ?-pinene, Mycerene, Carvacrol, Fenchone, ?-terpinene, ?-elemene, ?-terpinene, Thymol, Limonene, Trans-anethole, Camphor, Estragole, Camphene, Sabinine, Mycrene were detected in variety RF-205. Anethole was most abundant compound in both fennel germplasm lines which ranged from a minimum of 69.35 % to a maximum of 73.44 %.

Authors :
Chitrangda Yadav : Ph.D Research Scholar, School of Life Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India
C.P. Malik : Advisor (Academics), School of Life Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India