Screening of Eugenia caryophyllus Essential oil and their Fractions against Fungi causing Superficial Skin Infection in Human beings

By : Neetu Jain

Page No: 189-195

Abstract
Eugenia caryophyllus (commonly known as clove) belonging to the family Myrtaceae is an important medicinal plant. For present study clove flowering buds were used for oil extraction procedure. Oil was extracted through hydrodistillation method using Clevenger’s apparatus. Eugenol was found as major constituent of oil. Clove oil was screened for their antidermatophytic properties against selected dermatophytes through disc diffusion technique and by minimum inhibitory concentration determinations. The results of experiments revealed moderate to high level antidermatophytic activities against tested microorganisms. Maximum zone of inhibition 63.83±0.166 mm was reported against Microsporum gypseum. Maximum activity index (AI = 3.36) was reported against M. canis (IZ=47 mm). Candida albicans was found to be the most resistant pathogen while Trichophyton rubrum was found to be most susceptible one. Oil was further subjected in Buchii’s glass oven equipment for fractional separation. Three fractions were obtained at different temperature interval and labeled as CLI - CLIII. Maximum antidermatophytic potential was observed in case of CLI due to higher concentration of eugenol. MIC were ranged from 0.025 ?l/ml to 0.1 ?l/ml. Present study concluded that essential oil of Eugenia caryophyllus oil possesses strong antidermatophytic properties.

Author :
Neetu Jain : S.S. Jain Subodh P.G.(autonomous) College, Jaipur
 

DOI: https://doi.org/10.32381/JPSR.2019.35.02.6