Individual and Combined Effect of Drought and Salinity on Electrolyte Leakage, Relative Leaf water content and Lipid peroxidation in Brassica juncea sp. supplemented with Salicylic acid

By : Rajani Chauhan, Dheera Sanadhya

Page No: 171-182

Abstract
Salinity and Drought are the major abiotic factors that affect the membrane integrity. The main check points to evaluate the damage on cell membrane are electrolyte leakage (EL) and Relative Leaf Water Content (RLWC). The level of lipid peroxidation indicates the severity of damage. Our study was aimed at evaluating the effect drought, salinity and their combined stress on membrane integrity and response of salicylic acid in mitigating the stress. The experiment was carried out under in-vitro conditions where two sets of 7-d-old plants were exposed to three levels of water stress (5 , 10 and 15 % PEG), salt stress (50, 100 and 150 mM NaCl) and combinations (Combination (1) 2.5 % PEG + 25 mM NaCl; Combination (2) 5 % PEG + 50 mM NaCl; Combination (3) 7.5 % PEG + 75 mM NaCl), another set of seedlings were also supplemented with same stress conditions along with the application of SA (8 ?m). These stresses significantly reduced the RLWC and increased EL content. The molecules of lipid peroxidation MDA and H2 O2 content also showed the increased value in the mustard leaves. Toxic effects induced by these osmotic stresses were completely overcome by the exogenous application of SA

Authors :
Rajani Chauhan : Assistant Professor, Dept. of Plant Breeding and Genetics R.B. (P.G.) College, Agriculture Campus, Agra U.P. India
Dheera Sanadhya : Associate Professor, School of Life Sciences, SIILAS Campus, Jaipur National University, Jaipur (Rajasthan) India
 

DOI: https://doi.org/10.32381/JPSR.2019.35.02.4