Development and Validation of a GC-MS with SIM Method for the simultaneous estimation of 13 docosenamide, n-tetracosanol-1 and squalene from the leaf extracts of Wagatea spicata

By : Nandini Girish, Vikas Vaidya, Seema Sapale

Page No: 295-302

Abstract:
Wagatea spicata (Dalzell) Wight (Moullava spicata (Dalzell) Nicolson) is a plant of the western ghats of India. The plant has been popularly used in tribal medicine as a cure for respiratory and skin disorders in the form of its roots and stem bark respectively. [8,16] However, the folklore does not give much account of the benefits of the leaves of the plant. Three phytoconstituents have been isolated and characterized using advanced techniques like the Infra-Red (IR) spectroscopy, Gas Chromatography-Mass Spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR) spectroscopy from the said plant. [12,13]

In order to establish the additional quality parameters, the prime objective of the present work was to assess the phytochemical profile of the plant. Effort was made to quantify 13 docosenamide, ntetracosanol-1 and squalene from the leaf extracts of Wagatea spicata using GC-MS. A simple, precise and accurate GC-MS method has been established for simultaneous quantitation of three components (13 docosenamide, squalene and n-tetracosanol-1) from Wagatea spicata.

Authors:
Nandini Girish : Ramnarain Ruia Autonomous College, Matunga, Mumbai
Vikas Vaidya : Royal College of Science, Arts and Commerce, Mira Road, Thane
Seema Sapale : Kirti M Doongursee College, Dadar, Mumbai

DOI: https://doi.org/10.32381/JPSR.2020.36.1-2.38