Pollen Viability Study of Solanum erianthum D. Don

By : Soumi Ghosh, Tustu Mondal, Anjalika Roy

Page No: 261-265

Abstract :
Pollen viability tested in Solanum erianthum D. Don by stain tests and in vitro pollen germination to ascertain the effects of essential compounds on pollen germination for designing efficient seed setting. Three stain tests (TTC, acetocarmine and safranin) and different growth media test e.g. sucrose, boric acid and gibberellic acid (alone and in combination) were done for pollen viability. The flowers generally open at 04:30 to 07:00 AM, after which anther dehiscence takes place. TTC test was more reliable. Percentage of germination was low in sucrose or boric acid medium. Maximum germination and pollen tube development was in 25% sucrose solution with 100 ppm boric acid. The gibberellic acid added to the basal medium reduced the germination rate. Accurate nutrient medium is required for proper growth and development of pollen tube.

Authors :
Soumi Ghosh :Department of Botany, Visva-Bharati, Santiniketan- 731235, India
Tustu Mondal : Department of Biotechnology, St. Xavier’s College, Burdwan-713103, India
Anjalika Roy : Department of Botany, Visva-Bharati, Santiniketan- 731235, India

DOI: https://doi.org/10.32381/JPSR.2020.36.1-2.34