Callus Induction, Proliferation, Somatic Embryogenesis and Plant Regeneration in Gladiolus Communis L.

By : Peer Saffeullah , Neelofer Nabi

Page No: 205-209

Abstract:
Gladiolus communis L. is an ornamental herb belonging to family Iridaceae, which is widely used in cut-flower business. But multiplication rate of Gladiolus is very slow in natural conditions and is damaged by Fusarium corm rot. So, this study was aimed to design an efficient callus induction and plant regeneration protocol for Gladiolus. Callus was induced using bulb explants of Gladiolus when incubated on MS medium supplied with various concentrations of 2,4-D. Maximum callus proliferation was obtained in 0.5 mgL-1 2,4-D amended medium, but the proportion of callus proliferation showed a decreasing tendency at higher concentration (1-2 mgL-1 2,4-D). At lower concentrations (0.5 mgL-1 2, 4-D), the maximum callus proliferation was observed (1.10 g), while at 1.0 mgL-1the callus biomass was 0.9 g in 4 weeks after inoculating the initial mass of 0.3g of callus . So, high concentrations of 2, 4-D decreased the rate of callus formation. Auxins particularly, 2,4-D (1-2 mgL-1) is crucial for initiation of embryogenicalli with maximum embryogenic induction frequency of 70% in 0.5 mgL-1 2,4-D supplemented MS medium. The medium when supplemented with BAP either alone or in combination with NAA results in development of somatic embryos with globular and other stages. The maximum no. of embryos was observed in 0.5 mgL-1BAP and 0.5 mgL-1 NAA amended medium. Plants were regenerated on the BAP and NAA supplemented medium with maximum conversion frequency of 75% in 0.5 mgL-1 BAP and 0.2 mgL-1 NAA added medium. Rooting of regenerated plants was done on 0.5 mgL-1 IBA fortified half strength MS medium. So, this efficient protocol for callus induction, proliferation, somatic embryogenesis can be used for rapid propagation of Gladiolus communis.

Authors:
Peer Saffeullah :Department of Botany, Jamia Hamdard, New Delhi, India-110062
Neelofer Nabi : Department of Botany, University of Kashmir, Srinagar, Jammu and Kashmir, India-190006

DOI: https://doi.org/10.32381/JPSR.2020.36.1-2.27