Antioxidant activity of micro propagated Ocimum citriodorum

By : K. Santhi, P. Udhayaraja, R. Sengottuvel

Page No: 197-201

Abstract :
In vitro studies on micropropagation protoplast culture on Ocimum citriodorum and its antioxidant activity was investigated. The healthy plants of Ocimum citriodorum were collected in Kodaikanal were raised and maintained at Poonga Biotech Research Centre, Chennai. Maximum primary callus initiation (75 ± 5.0 %) for Ocimum citriodorum was documented in leaf explants on MS medium supplemented with 2, 4-D (4.52 ?M). Similarly MS medium supplemented with BA (4.44 ?M) and NAA (0.14 ?M) recorded 75 % shoot regeneration producing highest number of total shoots (8.33 ± 1.15) with an average shoot length of 5.43 ± 0.06 cm from nodal explants after six weeks of culture. the optimal rooting response was observed on half strength MS medium supplemented with IBA (2.46 ?M) in terms of average number of roots (6.0± 1.0) with mean root length of 4.90 ± 0.26 cm per shoot with 75±5.0 % of rooting response recorded after 30 days of culture. mother plant and in vitro plant had the highest radical-scavenging activity (71.3%) and (65.2) respectively. Least percentage of radical-scavenging activity (17.6 %) was recorded in the in vitro callus extract (Methanol extract) of Ocimum citriodorum.

Authors :
K. Santhi : Assistant professor, Department of Botany, Selvamm Arts and Science College, Namakkal
P. Udhayaraja : Assistant professor, Department of Microbiology, Kongu College of Arts and Science, Karur
R. Sengottuvel : Assistant professor, Department of Botany, Arignar Anna Govt Arts College, Namakkal

DOI: https://doi.org/10.32381/JPSR.2020.36.1-2.25