High Throughput Extraction, Partial Purification and Characterization of Antioxidant Enzyme Ascorbate Peroxidase (Apx) Present in Chilli (Capsicum Annuum, Var. Azad Mirch-1) Plant

By : Saumya Katiyar , Anil Kumar

Page No: 117-124

Abstract
In this study, extraction, partial purification and characterization of antioxidant enzyme ascorbate peroxidase (APX) present in chilli (Capsicum annuum, var. Azad mirch-1) plant was performed. Fresh tissues of chilli plant were grown in experimental form of the Rama University Mandhana, Kanpur (India). APX enzyme was isolated in vitro conditions and detected by the spectrophotometic based enzyme assay. APX was purified with the use of column chromatography. APX enzyme was purified up to 59.62 fold with 30.33 % yield. The native molecular weight was obtained 57 kDa (homomer). The optimal temperature and pH of the chilli APX enzyme were found 55 °C and 7.5. The Km values were determined for both the substrate i.e. ascorbate and H2 O2 by drawing plots between enzyme velocity vs substrate concentration and obtained Km values of ascorbate and H2 O2 were found 2.41 mM and 1.77 mM respectively. Present study may be helpful for the agromonic practices and health related issues.

Authors :
Saumya Katiyar: Ph.D. Scholar, Department of Biotechnology, Faculty of Engineering and Technology, Rama University, U.P., Kanpur (India)
Anil Kumar : Assistant Professor, Department of Biotechnology, Faculty of Engineering and Technology, Rama University, U.P., Kanpur (India)

DOI: https://doi.org/10.32381/JPSR.2020.36.1-2.15