Advancement of CRISPR-Cas9 Technology in Clinical Research

By : Bhawna Srivastava, Reddy P.B

Page No: 125-131

Abstract: CRISPR has emerged recently as a powerful molecular tool to quickly alter any mutated sequence of DNA in any gene accurately. Scientists have found it to be more efficient and accurate than other existing genome editing methods, that has the potential to transform genetic research and can greatly impact human health, food security, and environmental sustainability.
The present study is based on secondary data obtained from various data sources. Results reveal that the CRISPR-Cas9 system consists of a Cas9 enzyme and a guide RNA, which has become an indispensable alternative tool for producing targeted mutations. Owing to its relative simplicity and cost-effectiveness compared to other molecular clippers (ZFN and TALEN), the CRISPR-Cas9 method has been revealed to have an immense control on genetic studies regardless of the species variation. This revolutionary CRISPR technology has been successfully applied in the treatment of wide range of genetic diseases, and in the food and agriculture industries to achieve environmental sustainability.
In the present manuscript, we will briefly introduce the CRISPR/ Cas9 system and talk about the issues and concerns around the CRISPR system. Besides, their applications in the treatment of a wide range of genetic diseases, and future perspectives on the CRISPR system will be described. Despite its immense prospects, major issues including off-target activity, method of delivery, and ethical and regulatory issues still remain unresolved. In this paper, we explore the up-to-date evolution in the CRISPR–Cas9 technology and its applications in the clinical diagnosis, treatment and prevention of several common human diseases along with its current status in India.

Authors :
Bhawna Srivastava
Department of Zoology, DAV College, Kanpur, U.P, India.

Reddy P.B
Government PG College, Ratlam, M.P, India.
 

DOI: https://doi.org/10.32381/PROD.2021.62.02.3