Antibacterial Activity of Chitosan from Squid Pens (Loligo chenisis) against Erwinia Carotovora from Soft Rot Postharvest Tomato Fruit

By : Hoang Ngoc Cuong, Huynh Thanh Tung, Nguyen Cong Minh, Nguyen Van Hoa, Pham Thi Dan Phuong, Trang Si Trung

Page No: 319-330

Abstract
The antibacterial activity of squid pen chitosans (deacetylation degree (DD) of 91%) with Mw varying from 93-1740 kDa was tested on Erwinia carotovora that causes soft rot disease in tomatoes. Specifically the inhibition level of chitosan to bacterial colonies development (in vitro) and disease lesion on tomato fruits (in vivo) was evaluated. Sigma’s chitosan (Mw of 50-90 kDa with DD of 80%) was used as positive control. In vitro results showed that the antibacterial action of chitosan depended greatly on the Mw of chitosan. The chitosan with a low Mw exhibited a higher inhibition activity on Erwinia carotovora than higher molecular weight commercial chitosan. The minimum inhibitory concentration of the chitosan with a Mw of 93 kDa was 1.25% (w/v). High Mw chitosan (Mw > 1000 kDa) did not show any inhibition activity in vitro.

Authors :
Hoang Ngoc Cuong and Huynh Thanh Tung : Faculty of Biotechnology, Binh Duong University, Vietnam
Nguyen Cong Minh : Institute for Biotechnology and Environment, Nha Trang University, Vietnam
Nguyen Van Hoa, Pham Thi Dan Phuong and Trang Si Trung : Faculty of Food Technology, Nha Trang University, Vietnam