Effect of Chitosan Calcium phosphate Bead size on Scaffold Properties as they relate to Formation of Biphasic Constructs for Osteochondral Regeneration

By : Anuhya Gottipati , Steven Elder

Page No: 287-304

Abstract
Treatment of injuries to articular cartilage is challenging as the tissue has limited capacity of intrinsic healing due to lack of blood vessels. This study explores a biphasic approach to cartilage tissue engineering, whereby cartilage forms in vitro on top of a bone mineral deposited, collagen type 1 coated biodegradable composite chitosan-calcium phosphate (CHI-CaP) scaffolds. In the current study, we showed the importance of bead size for these CHI-CaP scaffolds. Bead size plays a crucial role in regulating the pore size, mechanical stiffness, neotissue formation, and also removal of waste products. The CHI-CaP scaffolds with three different bead sizes were fabricated and tested for pore size, mechanical stiffness, and tissue formation. CHI-CaP scaffolds were seeded initially with osteosarcoma cells and then with chondrocytes for cartilage regeneration. Results showed that the scaffolds made of 0.984±0.11 bead size with 34% porosity are ideal of cartilage tissue formation.

Authors :
Anuhya Gottipati and Steven Elder : Department of Agricultural and Biological Engineering, Mississippi State University, Starkville, MS, USA