Covalent Immobilization of Lipase on Novel Nanofibrous Membrane for Catalysis of the Organic Synthesis

By : Yinchun Fanga, Xinhua Liua B, XU Yanga, Cuie Wang

Page No: 111-119

Abstract
Enzymes are green biocatalysts which have been widely used in many fields. Immobilization enzymes on nanofibrous membrane possessed easy recycling and high stability which would broaden their applications. Covalent immobilization of lipase could endow them higher stability than other protocols. In this study, a novel nanofibrous membrane containing epoxy groups and hydrophilic polyethylene oxide branch was used as a support for lipase immobilization. The immobilized lipase was used as the biocatalyst to catalyse Rap. stroermer reaction. The results showed that it obtained the high product yield of 88% when the volume ratio of methanol and water was 4:1, the dosage of immobilized lipase was 40~50 mg, the reaction temperature and time were 30~35 °C and 10 h.

Authors :
Yinchun Fanga, Xinhua Liua B, XU Yanga, Cuie Wang
College of Textile and Clothing, Anhui Polytechnic University, Wuhu 241000, China
 

DOI: https://doi.org/10.32381/JPM.2019.36.02.1