Captive Brood-Stock Development, Induced Breeding, Histology and Nursery Rearing of Olive Barb, Puntius Sarana Sarana (Hamilton)

By : N. Basavaraja , Nityanaibedya Biswal , K. Pau Biak Lun

Page No: 155-169

Abstract
Puntius sarana sarana holds promise as a candidate for the diversification of freshwater aquaculture. To raise brood-stock, fingerlings/sub-adults were collected from a perennial reservoir, transported, acclimatized and reared in prepared ponds. On attaining maturity, the sex of brooders was determined based on morphological features. The captive brooders were first induced to breed with ovatide at 0.5-0.75 ml/ kg female and 0.15-0.25 ml/kg male. The interval between hormone injection and spawning ranged between 8 and 10 h at 26-28.5°C. The fertilization rate varied between 35.5 and 93%. The hatching period and hatching rate were 15-20 h and 98%, respectively. The eggs and embryos developed normally; the yolk-sac absorption period was 3-4 d. Subsequently, this species was also induced to spawn with ovaprim and the results compared with those of ovatide. Of the two spawning agents tested, ovatide was found to be marginally better than ovaprim. Histological examination showed the presence of immature, maturing and mature oocytes and post-ovulaotry follicle, whereas the testis comprised of spermatogonia, spermatocytes, spermatids and spermatozoa. The larvae reached a size of 1.45 g (3.7 cm) and 2.9 g (6.3 cm) after being reared for 25 and 55 d, respectively.

Authors :
N. Basavaraja, Nityanaibedya Biswal And K. Pau Biak Lun
Department of Aquaculture, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore-575002, India.