Genetic Differentiation of Field Isolates of Vibrio Alginolyticusand V.Parahaemolyticus by using RAPD Fingerprinting Pattern

By : N.P. Marhual , S. Sahu , B.K. Das , K.K Nayak

Page No: 71-84

Abstract
From 28 isolates grown on Thiosulphate-Citrate Bilesalt-Sucrose agar (TCBS) medium, seventeen yellow colour colonies representing one strain of V. alginolyticus and eleven green colour colonies representing one strain of V. parahaemolyticus were identified. These isolates obtained from different organs eg. external surface, haepatopancreas, pleopod and telson of diseased Penaeus monodon were selected for the genotyping using RAPD fingerprinting for molecular differentiation between V. alginolyticus and V. parahaemolyticus. One hundred primers belonging to Operon series A, B, C, G and H were used for amplifying the genomic DNA profile of the both bacterium. In V. alginolyticus, 48 primers were amplified where as 59 primers produced more than 2 bands in V. parahaemolyticus. Genomic DNA of both the species could only able to amplify 35 percent of primers. In total, series only 11 primers could amplify for V. alginolyticus and not for V. parahaemolyticus. Similarly 24 primers could amplify for V. parahaemolyticus not for V. alginolyticus. These primers could potentially be used for the genetic differentiation and strain identification of both the bacterium from the diseased shrimp of P. monodon.

Authors :
N.P. Marhual, B.K. Das and S. Sahu
Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-751002, India

K.K Nayak
KBDAV College Nirakarpur , Khorda