DNA Barcode of Indian Spiny Loach, Lepidocephalus Thermalis(valenciennes) 61-69 of Tamil Nadu

By : Jaculine Pereira. J , B. Ahilan , Karl Marx , Jeya Shakila. R , Rajagopalsamy. C.B.T.

Page No: 61-69

Abstract
The Indian spiny loach, Lepidocephalus thermalis was collected from various regions of Tamil Nadu. Morphometric and meristic characterization were carried out for L. thermalis and DNA was isolated from L. thermalis using phenol chloroform rapid non-invasive technique. Purity of DNA was checked with UV spectrophotometer at 260 nm and 280nm and DNA was confirmed with 1 percent Agarose gel Electrophoresis. DNA barcoding was carried out for the DNA isolates using Cytochrome C Oxidase subunit 1(COI) gene. Polymerase Chain Reaction (PCR) was carried with COI gene primer and the PCR product was confirmed with 2 percent Agarose gel electrophoresis using 100bp DNA ladder and DNA content was analysed with Biophotometer. PCR products were sequenced and submitted to Genbank (NCBI).

Authors :
Jaculine Pereira, J., B. Ahilan, K. Karalmarx, R. Jeyashakila and C.B.T. Rajagopalsamy
Fisheries College and Research Institute, Thoothukudi-628 008